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Glial Calcium Waves are Triggered by Seizure Activity and Not Essential for Initiating Ictal Onset or Neurovascular Coupling.

TitleGlial Calcium Waves are Triggered by Seizure Activity and Not Essential for Initiating Ictal Onset or Neurovascular Coupling.
Publication TypeJournal Article
Year of Publication2017
AuthorsBaird-Daniel E, Daniel AGS, Wenzel M, Li D, Liou J-Y, Laffont P, Zhao M, Yuste R, Ma H, Schwartz TH
JournalCereb Cortex
Volume27
Issue6
Pagination3318-3330
Date Published2017 06 01
ISSN1460-2199
Keywords4-Aminopyridine, Animals, Brain Mapping, Calcium, Calcium Signaling, Carbenoxolone, Diagnostic Imaging, Disease Models, Animal, Epilepsy, Evoked Potentials, Somatosensory, Male, Neuroglia, Neurons, Neurovascular Coupling, Potassium Channel Blockers, Rats, Rats, Sprague-Dawley, Sodium Channel Blockers, Somatosensory Cortex, Tetrodotoxin
Abstract

It has been postulated that glia play a critical role in modifying neuronal activity, mediating neurovascular coupling, and in seizure initiation. We investigated the role of glia in ictogenesis and neurovascular coupling through wide-field multicell and 2-photon single cell imaging of calcium and intrinsic signal imaging of cerebral blood volume in an in vivo rat model of focal neocortical seizures. Ictal events triggered a slowly propagating glial calcium wave that was markedly delayed after both neuronal and hemodynamic onset. Glial calcium waves exhibited a stereotypical spread that terminated prior to seizure offset and propagated to an area ~60% greater than the propagation area of neural and vascular signals. Complete blockage of glial activity with fluoroacetate resulted in no change in either neuronal or hemodynamic activity. These ictal glial waves were blocked by carbenoxolone, a gap junction blocker. Our in vivo data reveal that ictal events trigger a slowly propagating, stereotypical glial calcium wave, mediated by gap junctions, that is spatially and temporally independent of neuronal and hemodynamic activities. We introduce a novel ictally triggered propagating glial calcium wave calling into question the criticality of glial calcium wave in both ictal onset and neurovascular coupling.

DOI10.1093/cercor/bhx072
Alternate JournalCereb Cortex
PubMed ID28369176
PubMed Central IDPMC6433182
Grant ListDP1 EY024503 / EY / NEI NIH HHS / United States
R01 EY011787 / EY / NEI NIH HHS / United States
R01 MH101218 / MH / NIMH NIH HHS / United States
R01 MH100561 / MH / NIMH NIH HHS / United States