|Title||A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by F-positron emitting tomography and fluorescence.|
|Publication Type||Journal Article|
|Year of Publication||2020|
|Authors||Guo H, Kommidi H, Lekaye CC, Koutcher J, Judenhofer MS, Cherry SR, Wu AP, Akin O, Souweidane MM, Aras O, Zhu Z, Ting R|
|Date Published||2020 Apr 16|
PURPOSE: Knowing the precise flow of cerebrospinal fluid (CSF) is important in the management of multiple neurological diseases. Technology for non-invasively quantifying CSF flow would allow for precise localization of injury and assist in evaluating the viability of certain devices placed in the central nervous system (CNS).
METHODS: We describe a near-infrared fluorescent dye for accurately monitoring CSF flow by positron emission tomography (PET) and fluorescence. IR-783, a commercially available near-infrared dye, was chemically modified and radiolabeled with fluorine-18 to give [F]-IR783-AMBF. [F]-IR783-AMBF was intrathecally injected into the rat models with normal and aberrant CSF flow and evaluated by the fluorescence and PET/MRI or PET/CT imaging modes.
RESULTS: IR783-AMBF was clearly distributed in CSF-containing volumes by PET and fluorescence. We compared IR783-AMBF (fluorescent at 778/793 nm, ex/em) to a shorter-wavelength, fluorescein equivalent (fluorescent at 495/511 nm, ex/em). IR783-AMBF was superior for its ability to image through blood (hemorrhage) and for imaging CSF-flow, through-skin, in subdural-run lumboperitoneal shunts. IR783-AMBF was safe under the tested dosage both in vitro and in vivo.
CONCLUSION: The superior imaging properties of IR783-AMBF could lead to enhanced accuracy in the treatment of patients and would assist surgeons in non-invasively diagnosing diseases of the CNS.
|Alternate Journal||EJNMMI Res|
|PubMed Central ID||PMC7163004|
|Grant List||CA008748 / / NIH/NCI Cancer Center Support Grant P30 / |
CA160373 / / US Department of Defense Congressionally Directed Research Projects Special Emphasis Grant on Pediatric Brain Tumors /
EB013904 / / National Institute of Biomedical Imaging and Bioengineering K99/R00 /
P30 CA008748 / CA / NCI NIH HHS / United States
CA148971 / / National Institute of Health RC2 /