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A near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by F-positron emitting tomography and fluorescence.

TitleA near-infrared probe for non-invasively monitoring cerebrospinal fluid flow by F-positron emitting tomography and fluorescence.
Publication TypeJournal Article
Year of Publication2020
AuthorsGuo H, Kommidi H, Lekaye CC, Koutcher J, Judenhofer MS, Cherry SR, Wu AP, Akin O, Souweidane MM, Aras O, Zhu Z, Ting R
JournalEJNMMI Res
Volume10
Issue1
Pagination37
Date Published2020 Apr 16
ISSN2191-219X
Abstract

PURPOSE: Knowing the precise flow of cerebrospinal fluid (CSF) is important in the management of multiple neurological diseases. Technology for non-invasively quantifying CSF flow would allow for precise localization of injury and assist in evaluating the viability of certain devices placed in the central nervous system (CNS).

METHODS: We describe a near-infrared fluorescent dye for accurately monitoring CSF flow by positron emission tomography (PET) and fluorescence. IR-783, a commercially available near-infrared dye, was chemically modified and radiolabeled with fluorine-18 to give [F]-IR783-AMBF. [F]-IR783-AMBF was intrathecally injected into the rat models with normal and aberrant CSF flow and evaluated by the fluorescence and PET/MRI or PET/CT imaging modes.

RESULTS: IR783-AMBF was clearly distributed in CSF-containing volumes by PET and fluorescence. We compared IR783-AMBF (fluorescent at 778/793 nm, ex/em) to a shorter-wavelength, fluorescein equivalent (fluorescent at 495/511 nm, ex/em). IR783-AMBF was superior for its ability to image through blood (hemorrhage) and for imaging CSF-flow, through-skin, in subdural-run lumboperitoneal shunts. IR783-AMBF was safe under the tested dosage both in vitro and in vivo.

CONCLUSION: The superior imaging properties of IR783-AMBF could lead to enhanced accuracy in the treatment of patients and would assist surgeons in non-invasively diagnosing diseases of the CNS.

DOI10.1186/s13550-020-0609-3
Alternate JournalEJNMMI Res
PubMed ID32301036
PubMed Central IDPMC7163004
Grant ListCA008748 / / NIH/NCI Cancer Center Support Grant P30 /
CA160373 / / US Department of Defense Congressionally Directed Research Projects Special Emphasis Grant on Pediatric Brain Tumors /
EB013904 / / National Institute of Biomedical Imaging and Bioengineering K99/R00 /
P30 CA008748 / CA / NCI NIH HHS / United States
CA148971 / / National Institute of Health RC2 /